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maxpar phosphate buffered saline pbs  (fluidigm)


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    Structured Review

    fluidigm maxpar phosphate buffered saline pbs
    Maxpar Phosphate Buffered Saline Pbs, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maxpar+phosphate+buffered+saline+pbs/Maxpar+PBS/us12521393-2853-8-12
    Average 95 stars, based on 161 article reviews
    maxpar phosphate buffered saline pbs - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Labeling:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Incubation:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Article Title: Peripheral blood leukocyte signatures as biomarkers in relapsed ovarian cancer patients receiving combined anti-CD73/anti-PD-L1 immunotherapy in arm A of the NSGO-OV-UMB1/ENGOT-OV30 trial.
    Article Snippet: .. After incubating for 45 min at room temperature (RT), superfluous antibody was washed away with MaxPar CSB, then with MaxPar phosphate-buffered saline (PBS) (Cat. no. 201058; Standard BioTools), after which cells were permeabilized by a 10-min incubation in pure methanol (Cat. no. 32213; Sigma) at 20°C. ..

    Saline:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification
    Article Snippet: .. 4×10 6 cells/mL) and rinsed by centrifugation with Standard Biotools Maxpar ® phosphate-buffered saline (PBS), prior to subsequent fixation in 4% paraformaldehyde for 10 minutes at room temperature. .. Titrated concentrations of either rhodium or iridium intercalators (Standard Biotools’ 500μM rhodium Cell-ID TM or 125μM iridium Cell-ID TM , respectively) were then doped into each sample to form intercalation concentration ranges of 0.05 μM – 0.5 μM and 0.005μM – 0.05 μM, respectively.

    Article Title: Optimizing the Structure of a Pt Metal-Chelating Polymer to Reduce Nonspecific Binding for Mass Cytometry.
    Article Snippet: Nε-Boc-L-lysine and H-dap(Boc)OMe·HCl were purchased from Bachem. .. CTL anti-aggregate buffer, Gibco RPMI 1640 cell culture media, fetal bovine serum (FBS), Maxpar cell-staining buffer (CSB), Maxpar phosphate-buffered saline (PBS), fix & perm cell fixation and permeabilization buffer, formaldehyde, Ir intercalator, Rh-intercalator, EQ4 beads, C-buffer, R-buffer, peripheral blood mononuclear cells (PBMCs), as well as all Maxpar reagents were supplied by Standard BioTools Canada, Markham ON. .. Ultrapure water (18.2 MΩ·cm) was obtained using a Thermo Barnstead GenPure xCAD Ultrapure Water system.

    Article Title: Peripheral blood leukocyte signatures as biomarkers in relapsed ovarian cancer patients receiving combined anti-CD73/anti-PD-L1 immunotherapy in arm A of the NSGO-OV-UMB1/ENGOT-OV30 trial.
    Article Snippet: .. After incubating for 45 min at room temperature (RT), superfluous antibody was washed away with MaxPar CSB, then with MaxPar phosphate-buffered saline (PBS) (Cat. no. 201058; Standard BioTools), after which cells were permeabilized by a 10-min incubation in pure methanol (Cat. no. 32213; Sigma) at 20°C. ..

    Article Title: Cytotoxicity targeting chimeras for CCR2-expressing cells
    Article Snippet: Following a 24 hour incubation period, Protein Transport Inhibitor Cocktail (500×) (Invitrogen/eBioscience) was added to all wells and incubated at 37° C. for 2 hours. .. Samples were then centrifuged and washed twice with MaxPar phosphate-buffered saline (PBS) (Fluidigm). .. Cells were then incubated with a viability reagent (200 nM cisplatin-198, Fluidigm) diluted in MaxPar PBS for 5 min at room temperature, then washed twice in MaxPar Cell Staining Buffer (Fluidigm).

    Article Title: Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model
    Article Snippet: Freshly cut TMA slides underwent dewaxing, rehydration, and antigen retrieval for 48 min in a Ventana Discovery Ultra Autostainer (Roche Diagnostics GmbH) using CC1-buffer (pH 9). .. Slides were washed with a soap detergent and rinsed in hot water to remove the oil before they were transferred to a Coplin jar and washed with Maxpar H 2 O and then MaxPar phosphate buffered saline (PBS) (Fluidigm). .. To avoid non-specific binding, slides were blocked with 3% freshly made bovine serum albumin (BSA)/PBS buffer (Sigma–Aldrich/Merck).

    Article Title: Peripheral blood leukocyte signatures as biomarkers in relapsed ovarian cancer patients receiving combined anti‐ CD 73/anti‐ PD ‐ L 1 immunotherapy in arm A of the NSGO ‐ OV ‐ UMB1 / ENGOT ‐ OV 30 trial
    Article Snippet: .. Count the cells in each sample using your preferred cell counting method, using MaxPar phosphate-buffered saline (PBS) (Standard BioTools, Cat.No. .. 201058) to dilute and resuspend samples in case of excessive cell density (NOTE: The cell counting in this study was done electronically, using two 10μL aliquots from each sample on the CountessTM II automated cell counter (ThermoFisher Scientific, Cat.No.

    Staining:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Cytometry:

    Article Title: Mass cytometric single cell immune profiles of peripheral blood from acute myeloid leukemia patients in complete remission with measurable residual disease.
    Article Snippet: .. To allow for distinction between single cells, cell aggregates, and debris, DNA was labeled with iridium-191/193 by incubation in 0.1 nM Ir-nucleic acid intercalator (Standard BioTools) diluted in a buffer containing MaxPar phosphate buffered saline (PBS) (Standard BioTools) and 4% paraformaldehyde (PFA) (Alfa Aesar, 16% PFA, methanol-free) overnight at 4 C. Immediately before sample acquisition, cells were washed first in MaxPar Cell Staining Buffer, next in MaxPar Cell Acquisition Solution (CAS) (both from Standard BioTools) and then left pelleted until analysis on the mass cytometer. .. The cells were resuspended in CAS supplemented with a 1:10 dilution of the EQ Four Element calibration beads (Standard BioTools).

    Centrifugation:

    Article Title: Cell-type specific iron content regulation revealed by single-cell iron quantification
    Article Snippet: .. 4×10 6 cells/mL) and rinsed by centrifugation with Standard Biotools Maxpar ® phosphate-buffered saline (PBS), prior to subsequent fixation in 4% paraformaldehyde for 10 minutes at room temperature. .. Titrated concentrations of either rhodium or iridium intercalators (Standard Biotools’ 500μM rhodium Cell-ID TM or 125μM iridium Cell-ID TM , respectively) were then doped into each sample to form intercalation concentration ranges of 0.05 μM – 0.5 μM and 0.005μM – 0.05 μM, respectively.

    Cell Culture:

    Article Title: Optimizing the Structure of a Pt Metal-Chelating Polymer to Reduce Nonspecific Binding for Mass Cytometry.
    Article Snippet: Nε-Boc-L-lysine and H-dap(Boc)OMe·HCl were purchased from Bachem. .. CTL anti-aggregate buffer, Gibco RPMI 1640 cell culture media, fetal bovine serum (FBS), Maxpar cell-staining buffer (CSB), Maxpar phosphate-buffered saline (PBS), fix & perm cell fixation and permeabilization buffer, formaldehyde, Ir intercalator, Rh-intercalator, EQ4 beads, C-buffer, R-buffer, peripheral blood mononuclear cells (PBMCs), as well as all Maxpar reagents were supplied by Standard BioTools Canada, Markham ON. .. Ultrapure water (18.2 MΩ·cm) was obtained using a Thermo Barnstead GenPure xCAD Ultrapure Water system.

    Cell Counting:

    Article Title: Peripheral blood leukocyte signatures as biomarkers in relapsed ovarian cancer patients receiving combined anti‐ CD 73/anti‐ PD ‐ L 1 immunotherapy in arm A of the NSGO ‐ OV ‐ UMB1 / ENGOT ‐ OV 30 trial
    Article Snippet: .. Count the cells in each sample using your preferred cell counting method, using MaxPar phosphate-buffered saline (PBS) (Standard BioTools, Cat.No. .. 201058) to dilute and resuspend samples in case of excessive cell density (NOTE: The cell counting in this study was done electronically, using two 10μL aliquots from each sample on the CountessTM II automated cell counter (ThermoFisher Scientific, Cat.No.



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